Journal: Science Advances
Article Title: Blood-derived APLP1 + extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases
doi: 10.1126/sciadv.ado6894
Figure Lengend Snippet: ( A ) NTA-derived graphs showing size and concentration metrics of the brain tissue EVs. ( B ) Percentage-based size distributions of EVs, derived from the data presented in (A). ( C ) TEM images, at ×10,000 and ×20,000 magnifications, depicting EV morphology. Scale bars, 100 nm. Error bars in the figures delineate the SE of the mean (±SEM) from triplicate measurements of brain tissue EVs. ( D ) Western blot of APLP1 and EV markers in brain tissues and brain tissue EVs from five C57BL/6 mice. ( E ) ZetaView analysis of plasma-derived EVs in WT and Thy-1 GFP M line mice. Total EVs appear in light-scatter mode, GFP + EVs in 488-nm fluorescence mode, and APLP1 + EVs in 640-nm fluorescence mode. ( F and G ) EV size distribution in WT and Thy-1 GFP M line mice using ZetaView. ( H ) Average EV size ranges between 198 and 217.8 nm in both mouse types. ( I ) Immunostaining of APLP1 in plasma EVs from Thy-1 GFP M line mice, with white arrowheads highlighting APLP1 + GFP + EVs. Scale bars, 25 μm. All data were obtained from at least three mice.
Article Snippet: EVs were diluted in PBS (1-ml final volume) and examined under a ZetaView Nanoparticle Tracking Video Microscope (Particle Metrix, Inning, Germany).
Techniques: Derivative Assay, Concentration Assay, Western Blot, Clinical Proteomics, Fluorescence, Immunostaining